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Accueil > APPELS D’OFFRES > Stages, thèses, post-docs > Bourses de thèse > PROduction of new oLIGOgalacturonides (OGs) by enzymatic degradation : towards new elicitor activities ?

PROduction of new oLIGOgalacturonides (OGs) by enzymatic degradation : towards new elicitor activities ?

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The aim of the PROLIGO project is to produce novel OGs by the action of polygalacturonases (PGs), to identify these OGs and to test their effects on plant immunity.

The PROLIGO project will allow :
1. The production in heterologous system (yeast Pichia pastoris to have large amount of enzymes) of a diversity panel of PGs from plants (Marchantia polymorpha, Physcomitrella, Arabidopsis thaliana) or from fungi (Verticillium dahliae or Aspergillus aculeatinus).
2. The digestion of cell wall (cell wall of Marchantia polymorpha, Physcomitrella, Arabidopsis thaliana) or commercial pectins with these PGs to determine their mode of action. This will be realized by the identification, using a recently developed LC-MS/MS oligoprofiling approach (Voxeur et al., in revision PNAS), of the OGs released. This work will be realized in close collaboration with the analytical platform of the UPJV.
3. The identification of the most efficient OG to trigger plant immunity : Pools of OGs generated in 2 will be applied on various GUS reporter lines (pPR1 ::GUS, pPGIP ::GUS, pPAD3 ::GUS…). Following application of OGs, the expression of defense-related genes will be monitored using RT-qPCR and ROS production will be followed using spectrophotometric methods. In parallel, the effects of the pools of OGs on plant growth will be assessed using dark-grown hypocotyl as a developmental model. In collaboration, the capacities of the pools of OGs in inducing resistance against pathogens will be tested using various pathosystems.
4. The fractionation and purification of OGs from OG pools showing the best ability to trigger plant immunity and plant resistance. The effects of purified OG will be assessed using the approach described in 3.

In addition to determining the mode of action of enzymes, the PROLIGO project will allow the identification of potential novel molecules that could be used for plant protection as alternative of current chemical treatments.

Compétences / Pré-requis pour le (la) candidat(e) :
The candidate will have a master 2 in plant biology or biochemistry. The project will involve biochemistry (heterologous expression, protein purification, enzymology), plant biology and analytical chemistry.

Composition du dossier de candidature :
1- CV + lettre de motivation.
2- Relevés de notes des 3 dernières années d’études dont l’année de L3 ou équivalent.
3- Diplômes de la Licence & du Master ou équivalents
Les étudiants n’ayant pas encore validé leur M2 devront fournir :
- Relevé de notes du premier semestre du M2
- Lettre d’appréciation du responsable de stage
4- Lettre d’appréciation du responsable de la formation précisant le classement du candidat et l’effectif de la promotion.
5- Lettres de recommandation.

Le dossier est à envoyer avant le 27 mai 2019 à :
- corinne.pau-roblot@u-picardie.fr
- jean-marc.domon@u-picardie.fr
- jerome.pelloux@u-picardie.fr

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